技术说明:
GREEN 绿 染 料(10000 X in DMSO) 是 一 款 多 功 能 且易于使用的工具,凝胶电泳在用于检测核酸(DNA 和RNA),经由预电泳凝胶染色,样品预染色和后电泳凝胶染色。明亮的绿色荧光带,非常低的背景是其主要功能,并具有高灵敏度和非常低的毒性。GREEN 绿染色的核酸结合它在 254nm 和 488 纳米处能有效地激发;它也可以在 312 纳米激发但其灵敏度损失。检测可用来执行用于溴化乙锭同样的仪器溴化物和 SYBR® Green 绿凝胶染色如标准 UV 紫外透射仪(254 纳米),以及与 CCD摄像机成像系统或基于激光扫描仪选择 SYBR® Green滤光镜。
产品特点:
灵敏度:检测低至 100pg 皮克的 dsDNA 每一条带。
高对比度:明亮的绿色荧光,以及超低的背景。
安全:极低的毒性。
容易使用:替换您的试剂,用 Green 绿色染料按照下列中的一个:
- 预凝胶电泳染色的灵敏度高。
- 样品预染色的迁移效果佳。
- 后电泳凝胶染色对于特定方案的需求。
名称 | 包装 | 货号 |
GREEN STAIN | 0,5mL | NAGS068,0005 |
2×0,500mL | NAGS068,001 | |
GREEN STAIN LOADING DYE | 1×1mL | NAGS116,CLK01 |
5×1mL | NAGS116,CLK05 |
Versatile and easy-to-use negligeable toxicity tool for the detection of nucleic acids(DNA and RNA)in electrophoretic gels.
Three protocols allowed:
•Pre-electrophoresisgel staining(for high sensitivity)
•Sample pre-staining(for best DNA migration)
•Post-electrophoresisgel staininig
Excitationat 254 and 488 nm
Green Stain is a product line of reagents for the detection of nucleic acids(DNAandRNA)after electrophoresis in agarose gels.
Versatile and easy-to-use with negligeable toxicity for molecular genetics laboratories.
Available as two formats:
•Green Stain-10000X in DMSO
•Green Stain–DNA loadingdye
Two vials format:
Vial A: Green Stain – Dye
Vial B: Green Stain - Loading buffer
Features
• Very low toxicity
• Optimal signal to background ratio
• Ready-to-use kit: simple mix your DNA sample and load into the gel
• Track and stain your DNA at once
• Simple storage: after recostitution stable 4°C for 6 months
GREEN STAIN -suggestedimagers
Gel visualization
•standard UV transilluminator (254 nm)
•laser-or LED-based imaging system selecting the SYBR® Green filter (460-490)